No, this is all encouraging stuff! I love when people have questions, especially good ones like yours, so thanks. First off, I should say that most questions will be answered in the live action feature film, but I will do my best to explain here. The animated stories are intended to be self contained brief snapshots in the life of Dr. Shroud, reserving chronological plotlines and subplots for other mediums (aforementioned film, comics, etc.).
That said, here you go:
"Was Laura's mom turned into a zombie and if so was the mentioned infection spread by bite or something else? "
She was infected by a bite from the rampant vampire infection in Necropolis.
"Also this episode and the web site imply that Dr. Shroud is/was married; what happened to his wife ? "
Married, currently separated. She's in the feature script.
"There are certain questions about Necropolis that need to be expanded on. Also is it me or was it a bit dark outside for 5pm?"
Necropolis will be explained eventually. It's always dark at 5PM in Necropolis. Think of it as perpetually Fall.
Making characters move better nowadays. :)