The bigger negative of this submission is the audio levels - everything is too loud. The the voices should be the easiest to hear, but it's competing with the background music and sound effects. Additionally, there are audio cut offs, such as Knuckles line "You too new guy."
Coloring could be better, such as not having the human character wearing a hat with a VERY similar shade of green as the grassy hills in the background. Also, avoid clashing art styles, since I also noticed that you used sprite clouds in the background which don't fit with your own art style.
However, you clearly did try with this submission. The timing of the actions is fine, if a tired cartoon gag.
I would suggest looking up color theory and audio tutorials.