Don't get me wrong...
...this is constructive criticism. I love sprite animations, I love Phoenix Wrong, but this just doesn't get up to the bar set by Ashford Pride. The jokes you made weren't very laugh-out-loud funny, but you are on the right track. Try using less Weird Al and more movie and video game clips. I thought the best part of this was the Luigi reference. Also, boobs and their corresponding characters are always relevant. Also, please refrain from using annoying things, however annoying the character may be.
Oh, one last thing: the audio needs to sync better.