Big improvement--some tweaking would go a long way
I watched this about three times, once without any sound. Afterwards, I waited about a day and then watched the first episode only to come back and watch this one over again.
I've got to say it there's a huge improvement over ep. 1.
+ I love the fact that the spotlight follows the Mike across the screen. It's movement could be delayed a bit more. Still, it's a very nice touch. For the future, I'd like to see the spotlight actually be a radial with transparent edges rather than a huge translucent disk. You may also want to try adding a shadow to the back wall.
- It's a bit short. I'd suggest saving these up a bit more until you've got a solid minute and a half before submitting it. It's kind of hard to enjoy great stand up a few jokes at a time.
+/- I liked the tweened movement--but it also comes off to making the character look flat and stiff. If you changed your symbols for your arms and legs much like you have for the mouth and eyes, you can give more range to the types of movements your character can have.
+ I liked the facial hair--you don't look like a 16 year old anymore. xD