For those who keep getting stuck with 59 of 60 Virgo symbols, search the rug that says "Tiger Lounge Hotel" right in the middle of those words with your "Assassin". Most likely that is the one you're missing.
For everything else, just be sure to use the "Assassin" everywhere, and especially the first time you enter a room as some rooms you cannot re-enter so you forfeit any symbols you didn't take first time you entered. Use it on every part of every wall and piece of furniture. Be very thorough.
Getting all of those Virgo symbols was really the only irritating part of the game.