The tutorial should absolutely not count towards your final score. If you don't immediately understand the spotlight mechanic, you can penalize yourself so severely that not even a perfect run can save you.
Beyond that, good game.
At first i was a bit confuse by the game. Got a grip of it tho
It's annoying killing like forty ants in the tutorial because I didn't know that a large light sacrifices ants and I didn't know if my score of negative seventy-nine was going to reset. It's annoying to know where a termite is and have to just sit there and stare at the headache-inducing dancing waiting for the light to get small enough that you aren't sacrificing points. It's annoying that no matter what, at the smallest light setting you still lose 8 ants every time. I cant tell you any more because I stopped playing there.
Edit: I don't want to be disrespectful about it, because I absolutely respect what you are doing and I encourage you to continue doing it. That was just kind of...a raw and unfiltered log of my emotional experience playing the game.
This was a pretty fun time killer puzzle game (my best score was: 79)