Not bad for a mouse animation
Well, I've seen a lot worse animations gracing the pages of Newgrounds over the years, so I really can't have a go at you for trying. I can point you in the right direction on a few parts however, as you could use a slightly wider spectrum of sound effects, for example. One single sound for the actions sounds like you're moving from frame to frame of a comic strip.
When it comes to the flying and what looks like rocket power, perhaps some sort of facial expression is needed, as otherwise it looks too simple. I'd also suggest that you come up with a design of a flame, as at the moment, it looks as if you've just got a line of body propelling him upwards. It could be some sort of flame, so the best thing I could suggest was to make it a triangular flame, then add a smaller orange one inside and an even smaller yellow flame, to make it look more realistic. Having the size of this flame change smaller then bigger would also help to stop potential boredom for the viewers.
Finally, when the face with eye lasers gets "Mario stomped", perhaps have the guy fly overhead, cut the power and drop, before firing up again and flying away?
When you're drawing, try zooming in and using a slightly smaller tool, to maximise control. When you zoom out, the piece will look so much more detailed, it will surprise you :)
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