NO NO NO:
Please Analog-Stick, in future rather than saying what you 'think' or 'assume' is right, just say nothing at all.
Vexagon:
Layers are simply an abstraction of the flash IDE, it makes no difference to the compiled flash whether you have 1 layer, or 10 million layers, the layers are simply an abstraction to help with controlling the depths at which graphics are ordered, and in terms of any actionscript over the layers, will control the order of execution of the scripts.
In short, the answer is NO.